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This is a one day workshop introducing researchers to key concepts behind high-throughput sequencing projects  (otherwise known as Next Generation Sequencing)


Registration has closed for the Project Management Workshop to be held
on 5 April 2011.
If you are interested in attending a future Project Managment Workshop
please advise Susan.


This one day workshop is an introductory course to Next Generation Sequencing especially designed for NZ Researchers. It will cover the chemistry and issues of the different sequencing platforms and applications available now, and those coming in the near future. Computing and Bioinformatics, issues over hardware, software and computing skills are covered as well as   budgeting for NGS projects for funding applications.

This course is suitable for those researchers and postgraduate students beginning work using Next Generation Sequencing, as well as team leaders planning future research applications. It is also suitable for those researchers who have been using one platform in the past and wish to take advantage of the other types of NGS in mixed platform projects.

A number of Frequently Asked Questions will be raised in this workshop including:

  • What type of NGS is suitable for my project?
  • What are the biases, issues surrounding the type of sequencing I plan to use?
  • Is it best to use a server or desktop solution for my bioinformatics?
  • What software is currently the best / most popular / freely available / commercially available?
  • What skills are needed to run the analysis?
  • What ethical/genetic permissions are typically required?
  • Where are the closest NGS sequencing and bioinformatic services?
  • Where is the future (end of 2011, and further) for NGS?

There will be time for participants to raise questions and issues from their own projects and take advantage of the expert advice onhand.


Registration for this Workshop is capped at 50 participants. Registrations will be taken on a first-come, first served basis. Registration is confirmed when payment is received.

The cost to attend the one day workshop is NZ$325 +15% GST per registrant ($373.75 incl GST). The registration form includes optional transport from the airport to Te Manawa (leaving the airport at 9.40am) and from Te Manawa to the airport (leaving the workshop venue at 4.10pm). Should you wish transport to be arranged for you please indicate this on the registration form. The cost of the shuttle service is $9 each way.

Registration includes attendance at the one day Project Management Workshop, morning tea, lunch and afternoon tea. The registration fee includes course material as supplied.

The next Project Management Workshop is still to be scheduled. If you are intertested in attending the next Project Managament Workshop please let Susan know and she'll email you when the date is set.

The Programme

The Workshop will start at 10.10am, with morning tea served from 9.45am, and finish at 4pm. The programme is yet to be finalised but the following is an indication of what will be covered:

Basic Chemistries

  • Illumina
  • 454
  • SOLiD
  • which applications are suited for which platforms?

Applications and Data Type

  • genome sequencing and resequencing
  • exon sequencing
  • transcriptomics and digital expression
  • small RNA
  • epigenetics (CHIP-seq and methylation)
  • metagenomics
  • sequence length and coverage for each application

Bioinformatics and computing

  • how much fire power is needed for your project
  • staffing
  • computing (server/desktop)
  • software

Building a project plan and budgeting – examples and projects from participants

  • genomics
  • transcriptomics – building a transcriptome then counting
  • metagenomics
  • other applications upon demand

Getting more from your project - Tricks and Traps

  • targeted research questions
  • training in NGS genomics
  • mixing platforms to get the right data
  • keeping up with the times – the dynamic nature of NGS
  • data management

Prerequisite Knowledge Expected

No prerequisite knowledge of Next Generation Sequencing or genomics is required.

The Venue

Te Manawa is the local Palmerston North Museum in the middle of Palmerston North at 326 Main St. See map.


LesleyDr Lesley Collins has extensive experience in working with 'Solexa' (aka Illumina)-generated data and was instrumental in getting the Illumina Genome Analyser at Massey University up and running. Lesley is also familiar with sequence data generated by the 454 and SoLiD systems and the latest HiSeq technology from Illumina. Her hands-on approach has seen her tackle many of the Solexa-focused bioinformatics demands and she will share her experience and knowledge with you. Lesley was course-controller for the highly successful Massey Genome Service Workshops that ran in 2010.

Lesley is currently editing a book "RNA Infrastcructure and Networks" to be released in February.


For any questions regarding the Progamme for the Workshop please contact Lesley.
For any questions on the organisation of the Workshop please contact Susan.